ABSTRACT
Objective To observe the effects of curcumin (Cur) on vasculogenic mimicry (VM) and PI3K pathway in choroid melanoma cell line OCM-1 in vitro 3-dimentional culture.Methods Three-dimensional culture of OCM-1 cells was performed for the observation of VM formation and the influence of concentration gradient (0μmol · L-1,10 μmol · L-1,20 μmol · L-1,30 μmol · L-1,40 μmol · L-1) Cur on VM,and immunohis to chemistry staining was used to detect the expression of PI3K and EphA2 in OCM-1 cell line.Results Cur could markedly inhibit the formation of VM in choroid melanoma cell line OCM-1 in vitro 3-dimensional culture with a dose-dependent manner.Cur suppressed EphA2 and PI3K expression in a concentration-dependent manner,and the difference between groups with 0 μmol · L-1 Cur group was significant statistically (all P =0.000),but there was no significant difference between 30 μmol · L-1and40 μmol · L-1 group (EphA2,P=0.300;PI3K,P=0.188).Conclusion Cur can significantly inhibit VM formation in choroid melanoma cell line OCM-1,of which the mechanism may be related to the downregulation expression of EphA2 and PI3K,thus inhibiting PI3K signaling pathway.